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首页> 外文OA文献 >The N-Acylneuraminate Cytidyltransferase Gene, neuA, Is Heterogenous in Legionella pneumophila Strains but Can Be Used as a Marker for Epidemiological Typing in the Consensus Sequence-Based Typing Scheme ▿ †
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The N-Acylneuraminate Cytidyltransferase Gene, neuA, Is Heterogenous in Legionella pneumophila Strains but Can Be Used as a Marker for Epidemiological Typing in the Consensus Sequence-Based Typing Scheme ▿ †

机译：N-酰基神经氨酸胞嘧啶转移酶基因neuA在嗜肺军团菌菌株中是异源的，但可在基于共识序列的分型方案中用作流行病分型的标记 ▿ †

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摘要

Sequence-based typing (SBT) is the internationally recognized standard method for genotyping Legionella pneumophila. To date all strains of serogroup 1 (SG1) and some of SGs 2 to 14 yield a seven-allele profile and can be assigned a sequence type (ST). However, for some strains belonging to SGs 2 to 14, the targeted region of the neuA gene could not be amplified using the published standard primers. We determined the DNA sequence of a neuA gene homolog located in the lipopolysaccharide synthesis locus of strain Dallas-1E. By using newly designed degenerate consensus primers based on the neuA homolog in strains Dallas-1E, Philadelphia-1, Paris, Lens, and Corby, we were able to obtain DNA sequences for all 48 non-SG1 strains which were untypeable by the standard method. Our data show that the neuA gene is present in all L. pneumophila strains but differs significantly in some non-SG1 strains at both the DNA and amino acid levels. The new primers can be used to amplify and sequence the neuA gene in all strains and can substitute for the standard primers. This offers the possibility of assigning an ST to all strains of L. pneumophila.
机译：基于序列的分型（SBT）是国际公认的对军团菌嗜肺菌进行基因分型的标准方法。迄今为止，所有血清群1（SG1）和一些SGs 2至14的菌株都产生了七个等位基因图谱，可以指定为序列类型（ST）。但是，对于属于SGs 2至14的某些菌株，neuA基因的目标区域无法使用公开的标准引物进行扩增。我们确定了位于菌株Dallas-1E的脂多糖合成基因座中的neuA基因同源物的DNA序列。通过在Dallas-1E，Philadelphia-1，Paris，Lens和Corby菌株中使用基于neuA同源物的新设计的简并共有引物，我们能够获得所有48株标准方法无法分型的非SG1菌株的DNA序列。 。我们的数据表明，neuA基因存在于所有嗜肺乳杆菌菌株中，但在某些非SG1菌株中在DNA和氨基酸水平上均存在显着差异。新的引物可用于扩增所有菌株中的neuA基因并对其进行测序，并可替代标准引物。这提供了将ST分配给所有嗜肺乳杆菌的菌株的可能性。

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